Primer
Part:BBa_K4636027:Design
Designed by: An-Jie, Liu Group: iGEM23_NTHU-Taiwan (2023-10-11)
Design
RPA primers were designed according to the TwistAmp® DNA Amplification Kits Assay Design Manual. We should notice that primer size is important for RPA reaction. In addition, there are other tips for designing RPA primer. Here are the considerations :
(1) Primer size between 30 and 36 bps.
(2) Primer GC% between 20 % and 70 %.
(3) Primer Tm between 50°C and 100°C.
(4) Avoid continuously repeating sequences.
(5) Confirm that our product size is large enough (>200 bps).
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